Kevin Nee, PhD, received his doctorate from UCI: School of Medicine. He is currently in the UCI Medical Scientist Training Program (MSTP). His dissertation research under Kai Kessenbrock, PhD, entitled "Breast Pericytes, Fibroblasts, and Adipocytes Distinguished at Single Cell Resolution" focused on defining the metabolic and paracrine interactions of the breast microenvironment in the pathogenesis of breast cancer at single-cell resolution.
PUBLICATIONS
Kevin Nee*, Dennis Ma*, Quy H. Nguyen, Maren Pein, Nicholas Pervolarakis, Jacob Insua-Rodriguez, Yanwen Gong, Grace Hernandez, Hamad Alshetaiwi, Justice Williams, Maha Rauf, Kushal Rajiv Dave, Keerti Boyapati, Aliza Hasnain, Christian Calderon, Anush Markaryan, Robert Edwards, Erin Lin, Ritesh Parajuli, Peijie Zhou, Qing Nie, Sundus Shalabi, Mark A. Labarge, Kai Kessenbrock. “Preneoplastic stromal cells promote BRCA1-mediated breast tumorigenesis.” Nature Genetics. 2023 March 13;55(3)
Tapsi Kumar*, Kevin Nee*, Runming Wei*, Siyuan He, Quy Nguyen, Shanshan Bai, Kerrigan Blake, Yanwen Gong, Maren Pein, Emi Sei, Min Hu, Anna Casasent, Aatish Thennavan, Jianzhuo Li, Ken Chen, Benedikt Nilges, Nachiket Kashikar, Oliver Braubach, Bassem Ben-Cheikh, Hui Chen, Mediget Teshome, Brian Menegaz, Huma Javaid, Chandandeep Nagi, Jessica Montalvan, Robert Edwards, Delia Tifrea, Erin Lin, Ritesh Parajuli, Sebastian Winocour, Alastair Thompson, Bora Lim, Devon Lawson, Kai Kessenbrock, Nadya Nikulina, Tuan Tran, Nicholas Navin. “A spatially resolved single cell genomic atlas of the adult human breast.” Nature. In Revision
Stephanie J Hachey, Silva Movsesyan, Quy H Nguyen, Giselle Burton-Sojo, Ani Tankazyan, Jie Wu, Tuyen Hoang, Da Zhao, Shuxiong Wang, Michaela M Hatch, Elizabeth Celaya, Samantha Gomez, George T Chen, Ryan T Davis, Kevin Nee, Nicholas Pervolarakis, Devon A Lawson, Kai Kessenbrock, Abraham P Lee, John Lowengrub, Marian L Waterman, Christopher CW Hughes. “An in vitro vascularized micro-tumor model of human colorectal cancer recapitulates in vivo responses to standard-of-care therapy.” Lab on a Chip. 2021 Feb 19; 21(7)
Hamad Alshetaiwi, Nicholas Pervolarakis, Laura Lynn McIntyre, Dennis Ma, Quy Nguyen, Jan Akara Rath, Kevin Nee, Grace Hernandez, Katrina Evans, Leona Torosian, Anushka Silva, Craig Walsh, Kai Kessenbrock. “Defining the emergence of myeloid-derived suppressor cells in breast cancer using single-cell transcriptomics.” Science Immunology. 2020 Feb 21;5(44)
Kevin Nee, Quy H. Nguyen, Kai Kessenbrock. “Single Nuclei RNA Sequencing of Adipose Tissue.” Protocols.io. 2018 Sep 25. dx.doi.org/10.17504/protocols.io.tdwei7e
Quy H. Nguyen, Nicholas Pervolarakis, Kevin Nee, Kai Kessenbrock. Experimental Considerations for Single- Cell RNA Sequencing Approaches. Frontiers in Cell and Developmental Biology. 2018 Sep 4;6:108
Marisabel Oliveros-Etter*, Ziwei Li*, Kevin Nee, Linzi Hosohama, Joseph Hargan-Calvopina, Serena A. Lee, Prakash Joti, Juehua Yu, Amander T. Clark. “PGC reversion to pluripotency involves erasure of DNA methylation from imprinting control centers follower by locus-specific re-methylation.” Stem Cell Reports. 2015 Sep 8;5(3):337-49
Joseph Hargan Calvopina, Helene Cook, John J Vincent, Kevin Nee, Amander T. Clark. “The Aorta Gonad Mesonephros organ culture recapitulates 5hmC reorganization and replication-dependent and independent loss of cytosine methylation from the mouse germline.” Stem Cells Dev. 2015 Jul 1; 24(13): 1536-45
Marco Morselli, William A. Pastor, Barbara Montanin, Kevin Nee, Robert Ferrari, Kai Fu, Giancarlo Bonora, Liudmilla Rubbi, Amander T. Clark, Simone Ottonello, Prof. Steven E. Jacobsen, Matteo Pellegrini. “In vivo targeting of de novo DNA methylation by histone modifications in yeast and mouse.” eLIFE. 2015 Apr 7;4:e06205
Ziwei Li, Juehua Yu, Linzi Hosohama, Kevin Nee, Sofia Gkountela, Sonal Chaudhari, Ashley Cass, Xinshu Xiao, Amander T. Clark. “The Sm protein methyltransferase PRMT5, is not required for primordial germ cell specification in mice.” EMBO J. 2015 Mar 12; 34(6):748-58
William A. Pastor, Hume Stroud, Kevin Nee, Dubravka Pezic, Sergei Manakov, Serena Lee, Guillaume Moissiard1, Déborah Bourc’his, Alexei A. Aravin, Amander T. Clark, Steven E. Jacobsen “MORC1 represses transposable elements in the murine male germ line.” Nat Communications. 2014 Dec 12;5:5795
John J. Vincent, Yun Huang, Pao-Yang Chen, Suhua Feng, Joseph H. Calvopiña, Kevin Nee, Serena A. Lee, Thuc Le, Alexander J. Yoon, Kym Faull, Guoping Fan, Anjana Rao, Steven E. Jacobsen, Matteo Pellegrini, Amander T. Clark. “Stage-Specific roles for tet1 and tet2 in DNA demethylation in primordial germ cells.” Cell Stem Cell. 2013 Apr 4 ;12(4):470–478
PRESENTATIONS:
• Keystone Symposia on Molecular and Cell Biology—Cancer Immunotherapy: Decoding the Cancer Immunity Interactome. “Epithelial-Mesenchymal-Immune Interactomes Decoded by single cell RNA sequencing of preneoplastic breast tissue”. Kevin Nee, Kai Kessenbrock. 2022, Whistler, British Columbia, Canada
• The Ameican Association for Cancer Research (AACR). “A spatially resolved single cell atlas of the human breast”. Tapsi Kumar, Quy Nguyen, Kevin Nee, Shanshan Bai, Min Hu, Emi Sei, Anita Wood, Jie Wiley, Hui Chen, Alejandro Contreras, Mediget Teshome, Ken Chen, Bora Lim, Devon Lawson, Nicholas Navin, Kai Kessenbrock. Cancer Research. 2020 Aug 8. 80(16), San Diego, CA
• Center for Complex Biological Systems Retreat, “Elucidating fibroblast and pericyte plasticity and their roles in cancer using temporal single cell RNA sequencing of microphysiological cancer models”. Kevin Nee, Stephanie Hatchey, Chris Huges, Kai Kessenbrock. 2020, Virtual
• Center for Complex Biological Systems Retreat, “Understanding Cellular State Transition in the Mammary Gland Microenvironment from a Control Theory Perspective”. Kevin Nee, Sun Jin, Qing Nie, Kai Kessenbrock. 2019, Hollywood, CA
• UCI T32 Retreat. “Microenvironment regulation of pericytes and adipocytes in breast homeostasis and cancer”. Kevin Nee, Kai Kessenbrock. 2019, Los Angeles, CA
• CZI West Coast Science Retreat for Pilot Projects for a Human Cell Atlas. “Defining the Human Breast Cellular Ecosystem Using Cell Single Cell and Nuclei RNA sequencing.” Kevin Nee, Quy H. Nguyen, Kai Kessenbrock 2018, Asilomar—Monterey, CA
• UCI Medical Scientist Training Program Annual Retreat, “Breast Mesenchymal Stem Cells and Preadipocytes Defined by Single Cell RNA Sequencing” Kevin Nee, Kai Kessenbrock. 2019, Lake Arrowhead, CA
• UCI Medical Scientist Training Program Annual Symposium. “Metabolic and paracrine interactions of the breast stroma and epithelium in BRCA carriers” Kevin Nee, Kai Kessenbrock. 2018, Irvine, CA
• UCI Medical Scientist Training Program Annual Retreat. “Methylation status of the DAPK1 promoter during in vitro microglia differentiation”. Kevin Nee, Mathew Blurton-Jones. 2016, Lake Arrowhead, CA
• UCI Medical Scientist Training Program Annual Retreat. “PGC reversion to pluripotency involves erasure of DNA methylation from imprinting control centers follower by locus-specific re-methylation” Kevin Nee, Marisabel Oliveros-Etter*, Ziwei Li*, Linzi Hosohama, Joseph Hargan-Calvopina, Serena A. Lee, Prakash Joti, Juehua Yu, Amander T. Clark. 2015, Lake Arrowhead, CA
• NIH Individual National Research Service Award MD/PhD Fellowship (F30), Granted by the National Cancer Institute (2019-Present)
o Title: Investigating the role of pericytes as regulators of breast tissue homeostasis and cancer.
o Award Amount: $150,755
• Keystone Symposia on Molecular and Cellular Biology—Travel Scholarship (2022)
o Award Amount: $1200
• ARCS Scholarship, Granted by ARCS Foundation (2020-2022)
o Award Amount: $10,000
• Training Grant Fellowship (T32), Granted by the National Cancer Institute (2018-2019)
o Amount Awarded: $32,500
• Center for Complex Biological Systems Opportunity Award— 2018 and 2019
o Total Awards Amount: $24,000
• United States Collegiate Archery—All American (2013)
• Whitcome Fellow Scholarship (2013)
o Award Amount: $3000
• Biomedical Research Scholarship (2012)
o Award Amount: $3000